Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Ultrafast and absolute quantification of SARS-CoV-2 on food using hydrogel RT-LAMP without pre-lysisArticle Published on 2023-01-152022-11-16 Journal: Journal of hazardous materials [Category] SARS, 치료기술, [키워드] Absolute quantification acute respiratory syndrome amplification cold chain Cold chain food contact infection coronavirus faster Food industry hydrogel Hydrogel RT-LAMP isothermal amplification microbe offer POCT point-of-care quantification reproducibility reverse transcription RNA RNA extraction RT-LAMP SARS-CoV-2 sensitivity specificity stability Surveillance Ultrafast detection. virus [DOI] 10.1016/j.jhazmat.2022.130050 PMC 바로가기
Multiple ligation-Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2Article Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification Analysis Bacterial colorimetric detection Colorimetric sensing conditions coronavirus detection system diagnosing disease Diseases effective Genome isothermal isothermal amplification limit of detection Multiple PK-probe point-of-care polymerase probe Pyrophosphate recombinase polymerase amplification Recombinase polymerase amplification (RPA) RNA robust SARS-CoV-2 SARS-CoV-2. selective sensitivity [DOI] 10.1016/j.talanta.2022.123835 PMC 바로가기
Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome amplification amplify catalytic clinical sample coronavirus coronavirus disease COVID-19 develop Diagnosis gold standard highlighting HPV-16 limit of detection Nanozyme strips nontoxic nucleic acid nucleic acid amplification Nucleic acid detection pandemic POCT point-of-care point-of-care testing polymerase REcombinase recombinase polymerase amplification reliability RPA RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. subsequent TMB unique viral infections virus [DOI] 10.1016/j.bios.2022.114739 PMC 바로가기
RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variantSARS-CoV-2 Delta 변이체에서 R203M 돌연변이의 신속한 검출을 위한 RT-LAMP 분석Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Accuracy amplification analyses appear AUC clinical samples conserved COVID-19 COVID-19 vaccines Delta delta variant Delta variants detect determined by Diagnosis discrepancy dominant Efficiency facilitate genotyping infected individuals Mutation N gene positive sample Primers quantification quarantine R203M mutation Rapid rapid diagnosis. RT-LAMP RT-qPCR SARS-CoV-2 sensitivity sequence Sequencing sets of primer sets of primers specificity specimen Surveillance threshold variant variants [DOI] 10.1080/22221751.2022.2054368 PMC 바로가기 [Article Type] Article
High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCRArticle Published on 2022-11-222022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification applied coronavirus detect detection diagnoses gold standard Graphene Graphene oxide high sensitivity limit of detection molecular multiplex Multiplex qPCR pandemic pathogen detection polymerase chain primer qPCR qPCR assay reduce reference gene RNase P RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 gene SARS-CoV-2. sensitivity single-stranded DNA ssDNA target virus [DOI] 10.1016/j.aca.2022.340533 PMC 바로가기
Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2Article Published on 2022-11-152022-10-06 Journal: Sens Actuators B Chem [Category] 진단, [키워드] acute respiratory syndrome amplification approach caused coronavirus COVID-19 COVID-19 pandemic cross-contamination Detection limit diagnostics Dumbbell-type triplex molecular switch Epidemics Frame gold standard High-fidelity independent Logic-gate molecular Molecular assay N gene nucleic acid amplification ORF1ab probe recognizing Region RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. spacer [DOI] 10.1016/j.snb.2022.132579 PMC 바로가기 [Article Type] Article
Cas12a-assisted RTF-EXPAR for accurate, rapid and simple detection of SARS-CoV-2 RNAArticle Published on 2022-11-152022-11-15 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] amplification Analysis approach COVID-19 pandemic CRISPR CRISPR-Cas12a detect Detection limit Detection sensitivity Developing End-point fluorescence equipment identify increase intrinsic isothermal Isothermy molecular point-of-care reported RGB RTF-EXPAR SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. specificity [DOI] 10.1016/j.bios.2022.114683 PMC 바로가기